Alpine 直接探测相机

型号 1124, 1125

直接探测相机显著降低了冷冻电子显微术（cryo-EM）的门槛。

优点

Alpine^® 系列直接探测相机显著降低了冷冻电子显微术（cryo-EM）的门槛。在 80 – 200 keV 电镜上，Alpine 系列相机就能够实现高分辨率结构的采集，从而让常规的冷冻电镜研究得以在更多价格可承受的日常主力设备上开展，并将冷冻电镜这一技术的效益扩展到更大的研究群体。

两种型号可选，提供不同视场大小以匹配您的应用需求:

Alpine (1124) – 借助单电子计数能力，Alpine 将 80 - 200 keV 的筛样电镜变为数据采集电镜
Alpine Vista (1125) – 提供 5,760 x 4,092 像素的大视场范围 (FOV), 将您高性能电镜的成像效率发挥到极致

Delivers a detective quantum efficiency (DQE) up to 2.4-times the performance of scintillator-based cameras

更高的探测量子效率 (DQE)，2.4 倍于闪烁体相机的性能

Makes the 2D classification of small molecules possible while improving macromolecule reconstructions

使小分子的 2D classification 成为可能，同时提高大分子重构的质量

简化工作流程，更早发现样品瑕疵/失效，更快获得更高质量的结构

Real-time counting ensures there is no compromise between data collection speed and quality

实时电子计数，无需在数据采集速度和质量之间妥协

产品说明书

Alpine 直接探测相机

媒体库

沉浸于科学发现的世界——探索揭示微观奥秘的震撼显微图像，同时观看展示 Gatan 技术实际应用的视频与教程。

发现细节

Aldolase (100 kDa) resolved to 3.07 Å at 100 keV acquired with an Alpine camera on a Talos F200C (equipped with an SFEG) at 45kx (0.851 Å/pixel) using CDS at ~5.2 e-/pixel/s, 100 ms frames, 10 frames/s. Samples were prepared by manual plunging samples onto glow discharged Quantifoil UltrAuFoil grids with 1.2 μm holes spaced 1.3 μm apart. The study used a Gatan model 626 side-entry holder for all samples and Leginon for data acquisition. Data courtesy Gabe Lander, The Scripps Research Institute.

Apoferritin resolved to 2.7 Å at 100 keV

Apoferritin (550 kDa) resolved to 2.7 Å at 100 keV acquired with an Alpine™ camera on a Talos F200C (equipped with an SFEG) at 45kx (0.851 Å/pixel) using CDS at ~5.2 e-/pixel/s, 100 ms frames, 10 frames/s. Samples were prepared by manual plunging samples onto glow discharged Quantifoil UltrAuFoil grids with 1.2 μm holes spaced 1.3 μm apart. The study used a Gatan model 626 side-entry holder for all samples and Leginon for data acquisition. Data courtesy Gabe Lander, The Scripps Research Institute.

When combined with Latitude software, Alpine delivers high-quality data at the highest imaging throughputs from 100 – 200 keV.

Transthyretin resolved to 4.6 Å at 100 keV

Transthyretin (55 kDa) resolved to 4.6 Å at 100 keV acquired with an Alpine camera on a Talos F200C (equipped with an SFEG) using CDS at ~5.2 e-/pixel/s. The study used a Gatan model 626 side-entry holder for all samples and Leginon for data acquisition. Data courtesy Gabe Lander, The Scripps Research Institute.